![](gallery2/invitrodev/light/thumbs/harpoondevth.jpg)
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The invitro development of the harpoon in insect tissue culture medium. Cells were acetone/Triton-X 100 (Sigma) fixed and permeated, then stained with Sytox Green (Molecular Probes). |
![](gallery2/invitrodev/light/thumbs/autospore2th.jpg)
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In vitro autosporulation of vegetative stages of helicosporidia. Cells were acetone/Triton-X 100 (Sigma) fixed and permeated, then stained with 2mg/mL Calcofluor White MR2. |
TEM
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![](gallery2/invitrodev/tem/thumbs/deth.jpg)
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TEM of the dehiscement event. |
![](gallery2/invitrodev/tem/thumbs/harpoondev3th.jpg)
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In vitro development of the filament to vegetative cell. |
![](gallery2/invitrodev/tem/thumbs/autosporeth.jpg)
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Vegetative cell of helicosporidia cultured in vitro. |