Steinernema
brazilense Nguyen, Ginarte, Leite, Santo & Haracava, 2010
Summary: A new entomopathogenic nematode, Steinernema brazilense,
was isolated from a single soil sample collected from a natural forest in Mato
Grosso do Sul state, Brazil. S.
brazilense is
characterized morphologically by features of infective juveniles (IJ), males
and females. For the IJ, body length averaging 1157 (1023–1284) µm, distance
from anterior end to excretory pore 95 (87–102) µm, from anterior end to end of
esophagus 148 (139–153) µm, tail length 85 (80–104) µm, D% and E% values 63 (58–70) and 106
(95–118.0), respectively. Lateral field pattern variable; the formula for the
arrangement of ridges from head to tail is: 2, 4, 6, 8, 6, 2. For the male, the
diagnostic characters include spicule averaging 83 (75–89) µm; D% about 65; the ratio SW% about 192. The length of spicule head is
greater than width. Lateral field with one narrow ridge. First generation
females are characterized by the presence of a ventral postanal swelling. S.
brazilense is morphologically close to Steinernema
diaprepesi. It can be differentiated from S.
diaprepesi by its longer IJ body length (1157
vs 1002 µm), longer distance from anterior end to excretory pore (110 vs 75 µm),
a longer tail length (103 vs 83 µm); males of the new species with longer
spicule (83 vs 79 µm). The new species can be distinguished further from other
members of glaseri
group by
characteristics of ITS and D2D3 regions rDNA.