Steinernema boemarei Lee, Sicard, Skeie & Stock, 2009
Summary: A new entomopathogenic nematode, Steinernema
boemarei n. sp., is described from southern France. Morphological, molecular
(28S and ITS rDNA sequence data) and cross-hybridisation studies were used for
diagnostics and identification purposes. Both molecular and morphological data
indicate that the new species belongs to the ‘glaseri-group’ of Steinernema spp.
Key morphological diagnostic traits for S. boemarei n. sp. include the presence
of prominent deirids (cervical papillae) on adult males, the morphology of the spicules
and gubernaculum, and the arrangement of the
23 genital papillae of the first generation males. Additionally, morphometric
traits of the third-stage infective juvenile, including total body length (mean
1,103 µm), tail length (mean 86 µm), location of the excretory pore (mean 91 µm),
and D% (mean 63), E% (mean 106) and H% (mean 41) values are definitive. In
addition to these morphological characters, analysis of both 28S and ITS rDNA
sequences depict this Steinernema species as a distinct and unique entity.