NEM 6103 - Insect Parasitic Nematodes

Lecture Schedule


Fall Semester, 2006


Room 2217, Entomology & Nematology Building

Period 6:  12:50-1:40 Thursday

Instructor:  Dr. Khuong B. Nguyen



Date                            Lecture topic


August 24                     Introduction to Insect Parasitic Nematodes

August 31                     Historical review. Type of relationships between insects and nematodes

September 7                 Parasitic adaptation of the nematodes: External and internal parasitisms; How to find nematodes; How to confirm parasitism of a nematode; Safety of EPNs; Effect on beneficial insects

September 14               Nematode distribution and survey methods (Dr. R. McSorley)

September 21               Order Tylenchida: Families Allantonematidae, Sphaerulariidae, Iotonchidae, Parasitylenchidae, and Fergusobiidae. Example: Deladenus

September 28               Tylenchida. Thripinema (Kelly Sims)

October 5                    Mermithida, Aphelenchida, Oyurida

October 12                  Midterm examination

October 19                  Diplogasterida and Rhabditida: Steinernematidae and Heterorhabditidae

October 26                  Life cycle and pathogenicity

November 2                 EPN in the United States

November 9                 No class (Instructor is in Mexico)

November 16               Strategies for infection of host: Ambusher, Cruiser, Continuum, Movement and Survival

November 23               Thanksgiving Holiday (no class)

November 30               Significance of insect parasitic nematodes

December 7                 Phylogeny - Review

                                    Final examination:



NEM 6104-L - Insect Parasitic Nematodes

Laboratory Schedule


Fall Semester, 2006


Room 2220-2217, Entomology & Nematology Building

Period 7-8: 1:55-3:50 Thursday



Date                            Lab number


August 24                     1 - Conduct a survey of the “Natural Area” for entomopathogenic nematodes (EPNs), and collect bark beetles for the lab. Set up traps. Students will stop by the lab to check for emergence of nematodes every day.

August 31                     2 - Observe different forms of nematodes from different insects: Beetles, cockroaches, crickets, Galleria mellonella (from baiting method).

September 7                 3 - Insect rearing (Nancy Loman, USDA 374-5801).

September 14               4 - Nematode rearing. Each group of students set up a chamber to rear nematodes. Steinernema glaseri will be used. Student presentation.

September 21               5 - Pathogenicity test: Galleria, fall army worms, cut worms; Nematodes used: Heterorhabditis zealandica, S. glaseri. Student presentation.

September 28               6 - Fergusobia (RobinGiblin-Davis).

October 5                    7 - Life cycle study: Dissect Galleria 1, 2, 3, 4 days after being exposed to S. glaseri. Look under a compound microscope. Described nematode stages observed. Student presentation.

October 12                  8 - Survival test: Release nematodes in soil of 5 containers, and check survival after 2 weeks by baiting technique. Student presentation.

October 19                  9 - Identification of nematodes using morphological characters.

October 26                  10 - Identification (continued). Get results for survival test. Student presentation.

November 2                 11 - DNA extraction. Student presentation.

November 16               12 - PCR process

November 23               Thanksgiving Holiday (no class)

November 30               13 - Purify DNA for sequencing.

December 7                 14 - Working with DNA sequences