NEM 6103 -
Insect Parasitic Nematodes
Lecture Schedule
Fall Semester, 2006
Room 2217,
Period 6: 12:50-1:40 Thursday
Instructor:
Dr. Khuong B. Nguyen
Date
Lecture topic
August 24 Introduction to Insect Parasitic Nematodes
August 31 Historical review. Type of relationships between insects and nematodes
September 7 Parasitic adaptation of the nematodes: External and internal parasitisms; How to find nematodes; How to confirm parasitism of a nematode; Safety of EPNs; Effect on beneficial insects
September 14 Nematode distribution and survey methods (Dr. R. McSorley)
September 21 Order
Tylenchida: Families Allantonematidae, Sphaerulariidae, Iotonchidae,
Parasitylenchidae, and Fergusobiidae. Example:
Deladenus
September 28 Tylenchida. Thripinema (Kelly Sims)
October 5 Mermithida, Aphelenchida, Oyurida
October 12 Midterm
examination
October 19 Diplogasterida and Rhabditida: Steinernematidae and Heterorhabditidae
October 26 Life cycle and pathogenicity
November 2 EPN in
the
November 9 No class (Instructor is in
November 16 Strategies for infection of host: Ambusher, Cruiser, Continuum, Movement and Survival
November 23 Thanksgiving Holiday (no class)
November 30 Significance of insect parasitic nematodes
December 7 Phylogeny - Review
Final examination:
NEM 6104-L -
Insect Parasitic Nematodes
Laboratory Schedule
Fall Semester, 2006
Room 2220-2217,
Period 7-8: 1:55-3:50 Thursday
Date
Lab number
August 24 1 - Conduct a survey of the “Natural Area” for entomopathogenic nematodes (EPNs), and collect bark beetles for the lab. Set up traps. Students will stop by the lab to check for emergence of nematodes every day.
August 31 2 - Observe different forms of nematodes from different insects: Beetles, cockroaches, crickets, Galleria mellonella (from baiting method).
September 7 3 - Insect rearing (Nancy Loman, USDA 374-5801).
September 14 4 - Nematode rearing. Each group
of students set up a chamber to rear nematodes. Steinernema glaseri will be used. Student presentation.
September 21 5 - Pathogenicity test: Galleria, fall army worms, cut worms;
Nematodes used: Heterorhabditis zealandica, S. glaseri. Student
presentation.
September 28 6 - Fergusobia (RobinGiblin-Davis).
October 5 7 - Life cycle study:
Dissect Galleria 1, 2, 3, 4 days after being exposed to S. glaseri. Look under a compound microscope.
Described nematode stages observed. Student presentation.
October 12 8 - Survival test: Release
nematodes in soil of 5 containers, and check survival after 2 weeks by baiting
technique. Student presentation.
October 19 9 - Identification of nematodes using morphological characters.
October 26 10 - Identification
(continued). Get results for survival test. Student presentation.
November 2 11 - DNA extraction. Student
presentation.
November 16 12 - PCR process
November 23 Thanksgiving Holiday (no class)
November 30 13 - Purify DNA for sequencing.
December 7 14 - Working with DNA sequences