Dov Borovsky

E-mail: dobo@ifas.ufl.edu

(100% Research)

1. Education:

•  B.A.,   University of California , Los Angeles , CA (Bacteriology), 1967

•  Ph.D., University of Miami , Miami , FL   (Biochemistry), 1972           

2. Employment and Academic Appointments:

• Research Foundation Professor (2000-present) Department of Entomology and Nematology, University of   Florida – IFAS.
• Professor (1990-present) Department of Entomology and Nematology, University of   Florida – IFAS.
• Associate Professor (1983-1990) Department of Entomology and Nematology, University of   Florida – IFAS.
• Research Biochemist (1978-1983) Florida Medical Entomology laboratory, Vero Beach
• Chemist III (1975-1978) Florida Medical Entomology laboratory, Vero Beach
• Research assistant professor 1975 University of Miami School of Medicine, Miami FL
• Research Instructor (1973-1975) University of Miami School of Medicine , Miami FL
• Post graduate research biochemist (1972-1973) University of California School of Medicine, Davis CA
• Teaching assistant (1967-1972) University of Miami School of Medicine, Miami FL

3. Responsibilities:

• Research and teaching in insect biochemistry, physiology and molecular biology of hormonal control of insects. Specifically, to use molecular and bioengineering approaches that have a potential in developing new biorational insecticides that can be expressed in algae, bacteria, yeast and plant cells and viruses.   These novel approaches are used to control mosquito larvae, as well as, agricultural pest insects, and provide foundation for future insect control.

4.   Accomplishments:

Honors

• NIH Career Development Award (1979-1983).
• Lady Davis Visiting Professorship Award in Biochemistry to the Hebrew University of Jerusalem (1986).
• Katholieke Universiteit , Zoological Institute, Leuven , Belgium Visiting Professorship Award in Biochemistry and Molecular Biology (1995).         
• Florida Entomological Society 1998 Achievement Award for research.
• Secretary of Agriculture Honor award (1999)
• Secretary of Agriculture Silver plow medal (1999)
• University of Florida .   Research Foundation Professorship (2000).

Appointments in Universities and Industry

•  Adjunct Associate Professor (1985-1991) University of Miami School of Medicine, department of biochemistry and molecular biology

•  Adjunct Professor (1991-present)   ) University of Miami School of Medicine, department of biochemistry and molecular biology

•  Visiting professor of Insect Biochemistry and Molecular Biology (1999-present) Katholieke Universiteit , Zoological Institute, Leuven , Belgium

•  Head of the scientific advisory board and consultant to Insect BioTechnology , Inc. (1993-2005) Durham North Carolina

Editorial Boards

• Archives of Insect Biochemistry and Physiology 1986-present
• Journal of Medical Entomology, December 1987-1993, Chairman, December 1988-1989, December 1992-December 1993.

Symposia organizer

• Co-organizer with professor Andrew Spielman of Harvard School of Public Health an international symposium entitled   "Host Regulated Developmental Mechanisms in Vector Arthropods" that convened in Vero Beach on February 3-6, 1986 , February 13-16, 1989 and February 8-11, 1993 .

Presentations at Scientific Meetings

• National meetings: 82 (80% by invitation)
• International meetings: 76 (all by invitation )

5. Grants: ($2,950,330 total costs, 1983-2004)

Recent Grants (past six years)

• TMOF in Chlorella: A new mosquito larvicide, NIH, ( 06/02/98 - 05/31/99), $100,000.  
• Biochemistry and physiology of insect peptide hormones, NATO ( 08/07/97 - 08/06/00), $7,495.00.  
• Sequencing and expressing TMOF receptor: a potential test to detect new insecticides that can block trypsin biosynthesis in insects midgets, Insect Biotechnology/Bayer (06-01-98-07-01-2000), $200,000.    
• Development of recombinant chlorella and yeast that carry TMOF as natural mosquito larvicides, Insect Biotechnology (06-01-98-07-01-99), $168,750.
• Using transgenic anabaena and chlorella sp. as potential mosquito larvicide, U.S.-Israel Binational Science Foundation ( 09-01-98 - 09-01-2001 ), $150,000.    
• Development of recombinant chlorella and yeast that carry TMOF as a natural mosquito larvicide, Insect Biotechnology ( 07-01-99 - 07-01-2000 ), $300,000.  
• Expression of TMOF: A new mosquito larvicide, NIH ( 06/02/00 - 05/31/03), $500,000.
• Development of recombinant chlorella and yeast that carry TMOF as a natural mosquito larvicide Insect Biotechnology ( 07-01-00 -   07-01-01 ), $300,000.  
• Diaprepes special grant funding.   Control of diaprepes by hormonal interdiction of digestion, USDA ( 09-01-00 - 09-01-05 ), $250,000.  
• Development of recombinant chlorella and yeast that carry TMOF as a natural mosquito larvicide, Insect Biotechnology ( 07-01-01 -   07-01-03 ), $250,000.  

6. Career Publications:

• Books (editor): 3
• Refereed papers: 67
• Non-Refereed papers: 31
• Chapters in books: 20
• Patents: 12
• Book reviews: 2
• Editorial: 1

•    Selected publications (since   1994):

• Borovsky, D., Carlson, D. A. Ujváry , I. and Prestwich , G. D.   1994.   Biosynthesis of (10 R )-juvenile hormone III from farnesoic acid by Aedes aegypti ovary.   Arch. Insect Biochem Physiol .   27: 11-25.
• Borovsky, D., Song, Q., Ma, M. and Carlson, D. A.   1994.   Biosynthesis, secretion and cytoimmunochemistry of trypsin modulating oostatic factor of Aedes aegypti.   Arch. Insect Biochem . Physiol . 27: 27-38.
• Carlson, D. A., Krueger, C. M., Hehman , G., Borovsky, D., Rossi, A. M., Cockburn, A. F., Shabanowitz , J. and Hunt, D. F.   1994.   Cloning and partial sequencing of the trypsin modulating oostatic factor gene from mosquito genomic libraries, and expression of the TMOF gene in E. coli , pp. 626-630 in ( Davey , K. G., Peter, R. E. and Tobe , S. S., editors) Perspectives in Comparative Endocrinology.    National Research Council Canada, Ottawa.  
• Borovsky, D., Carlson, D. A., Hancock, R. G., Rembold , H and Van Handel, E. 1994.   De novo biosynthesis of juvenile hormone III and I by the accessory glands of the male mosquito: organ culture and mass spectra evidence.   Insect Biochem . Mol. Biol.   24: 437-444.
• Bylemans , D., Borovsky, D., Hunt, D. F., Grauwels , L., and DeLoof , A.   1994. Sequencing and characterization of oostatic factor (TMOF) from the ovaries of the grey fleshfly Neobellieria ( Sarcophaga ) bullata .   Regulatory Peptides 50: 61-72.
• Borovsky, D., Powell, C. A., Nayar, J. K., Blalock, J. E., and Hayes, T. K. 1994.   Characterization and localization of mosquito-gut receptors for trypsin modulating oostatic factor (TMOF) using a complementary peptide and immunocytochemistry.   FASEB J.   8:350-355.
• Armstrong, P., Borovsky, D., Shope , R. E., Morris, C., Mitchell, C., Karabatsos, N., and Spielman , A.   1995.   A sensitive and specific colorimetric assay for the detection of eastern equine encephalomyelitis viral RNA in mosquitoes by the dot polymerase chain reaction.   J. Med. Ent . 32: 42-52.
• DeLoof , A., Bylemans , D., Schoofs , L., Janssen, I., Spittaels , K., Vanden Broeck , J., Huybrechts , R., Borovsky, D., Hua , Y., Koolman , J., and Sower , S. A.   1995.   Folliculostatins , gonadotropins and a model for control of growth in the grey fleshfly , Neobellieria   ( Sarcophaga ) bullata .   Insect Biochem . Mol. Biol.   25:661-667.
• Bylemans , D., Proost , P., Samijin , B., Borovsky, D., Grauwels , L., Van Damme , J., Van Beeumen , J. and DeLoof , A.   1995.   Neb- colloostatin , a second folliculostatin of the grey fleshfly , Neobellieria bullata .   Eur . J. Biochem .   228:45-49.
• Bylemans , D., Hua , Y-J., Chiou , S-J., Koolman , J., Borovsky, D. and DeLoof , A.   1995.   Pleiotropic effects of trypsin modulating oostatic factor (Neb-TMOF) of the fleshfly Neobellieria bullata .   Eur . J. Entomol .   92:143-149.
• Borovsky, D. and Mahmood , F.   1995.   Feeding the mosquito Aedes aegypti with TMOF and its analogs, effect on trypsin biosynthesis and egg development. Reg. Peptides 57: 273-281.
• Bylemans , D., Verhaert , P., Janssen, I. , Vanden Broeck , J., Borovsky, D., Ma, M. and DeLoof , A.   1996.   Immunolocalization of the oostatic and prothoracicostatic peptide, Neb-TMOF, in the fleshfly Neobellieria bullata .   Gen. Comp. Endocrinol . 103: 273-280.
• Borovsky, D., Janssen, I. , Vanden Broeck , J., Huybrechts , R., Verhaert , P., DeBondt , H. L., Bylemans , D. and DeLoof , A.   1996.   Molecular sequencing and modeling of Neobellieria bullata trypsin : Evidence for translational control with Neb TMOF.   Eur . J. Biochem .   237 (1): 279-287.
• Bylemans , D., Borovsky, D., Ujváry , I. and DeLoof , A.   1998.   Biosynthesis of juvenile hormone III, juvenile hormone III bisepoxide , and methylfarnesoate during the reproductive cycle of the grey fleshfly , Neobellieria ( Sarcophaga ) bullata .   Arch, Insect Biochem . Physiol . 37: 248-256.
• Borovsky, D., Powell, C. R., Dawson, W. O., Shivprasad , S., Lewandowski, D., DeBondt , H. L., DeRanter , C., and DeLoof , A.   1998.   Trypsin modulating oostatic factor (TMOF): A new biorational insecticide against mosquitoes.   Pp. 131-140 in Insects, Chemical Physiological and Environmental Aspects (eds. D. Konopinska , G. Goldsworthy, R. J. Nachman , J. Nawrot , I. Orchard, G. Rosinski ), University of Wroclaw 1997.
• Chiou , S.-J., Broeck , J. V., Janssen, I. , Borovsky, D., Vandenbussche , F., Simonet , G., and De Loof , A.   1998.   Cloning of the cDNA encoding Scg -SPRP, an unusual ser-protease-related protein from vitellogenic female desert locust ( Schistocerca gregaria ).   Insect Biochem . Molec . Biol. 28: 801-808.
• Xin-Hua Yan , DeBond , H.   L., Powell, C.C.,   Bullock, R. C., and Borovsky, D.   1999.   Sequencing and characterization of the citrus weevil Diaprepes abbreviatus , trypsin cDNA ; effect of Aedes trypsin modulating oostatic factor on trypsin biosynthesis.   Eur . J. Biochem .   262 (3): 627-636.
• Gelman , D. B. and Borovsky, D. 2000.   Aedes aegypti TMOF modulates ecdysteroid production by prothoracic glands of the gypsy moth Lymantria dispar .   Arch. Insect. Biochem . Physiol .   45: 60-68.
• Nauen , R., Sorge , D., Sterner, A., and Borovsky, D.   2001.   TMOF like factor controls the biosynthesis of serine proteases in the larval gut of Heliothis virescens .   Arch. Insect Biochem . Physiol .   47:169-180.
• Borovsky, D. 2003.  Trypsin Modulating Oostatic Factor: A Potential New Larvicide for Mosquito Control. J. Experimental Biol. 206: 3869-3875.

University of Florida Entomology and Nematology Dept. |  Florida Medical Entomology Laboratory (Vero Beach)